universal methylated human dna standard Search Results


human methylated dna  (Zymo Research)
95
Zymo Research human methylated dna
Human Methylated Dna, supplied by Zymo Research, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bisulfite converted universal methylated human dna standard  (Zymo Research)
93
Zymo Research bisulfite converted universal methylated human dna standard
Bisulfite Converted Universal Methylated Human Dna Standard, supplied by Zymo Research, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cpgenome human methylated dna controls  (Merck KGaA)
90
Merck KGaA cpgenome human methylated dna controls
Cpgenome Human Methylated Dna Controls, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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universal methylated human dna standard  (Enzo Biochem)
90
Enzo Biochem universal methylated human dna standard
CSB depletion by histone H3 hypoacetylation of its promoter. a Quantitative RT-qPCR of DNMT3A , DNMT3B , and DNMT1 . b <t>DNA</t> methylation (percent) of eight individual CpG sites in the CSB promoter at the indicated PN (positive control [CTL + ], a universal methylated (human) DNA standard). n = 3 independent experiments, mean ± SD; two-way ANOVA ( F = 2.983, DFn = 5, DFd = 96, p = 0.0151) with post-hoc Tukey’s test. c Immunoblots of H3 acetylated (H3Ac) and total histone H3 (reprobed after H3Ac stripping) at the indicated PN, with GAPDH as a loading control. d Quantitative RT-qPCR of HDAC1 and HDAC2 . e Quantitative PCR of DNA in a ChIP assay with either α-H3 acetylated or total α-H3 (material not available at PN35). Primers detect the occupancy of H3Ac/H3 at a specific region of the CSB promoter. Results are expressed as the percentage of input DNA normalized to H3 occupancy. f Scheme indicating the anacardic acid (AA) target. g Quantitative RT-qPCR of CSB upon treatment with increasing concentrations of AA or DMSO (Vehicle) for 24 h, and in untreated control (Untreated). h Immunoblot of H3 acetylated, total H3 histone, CSB, HTRA3, HTRA2, and p21 from whole-cell extracts with increasing concentrations of AA or DMSO (Vehicle) for 24 h, and 24 h after AA withdrawal and in the corresponding controls (Untreated). Samples on the same blot are framed; each frame displays the respective GAPDH used as a loading control (GAPDH (F-C staining in the middle blot)). Quantitative PCRs: n = 3 independent experiments; mean ± SD; one-way ANOVA ( a DNMT1 : F = 9.129, DFn = 5, DFd = 12, p = 0.0009; DNMT3A : F = 0.05094, Dfn = 5, DFd = 12, p = 0.9980; DNMT3B : F = 0.5325, DFn = 5, DFd = 12, p = 0.7481; d HDAC1 : F = 12.93, DFn = 5, DFd = 12, p = 0.0002; HDAC2 : F = 3.826, DFn = 5, DFd = 12, p = 0.0264; e H3: F = 2.298, DFn = 4, DFd = 10, p = 0.1304; H3Ac/H3: F = 5.064, DFn = 4, DFd = 10, p = 0.0171; g F = 9.516, DFn = 4, DFd = 10, p = 0.0019) with post-hoc Tukey’s test vs. PN16 (or Vehicle, g ) when not specifically indicated. i Quantitative PCR analysis of a DNA fragment in the CSB promoter from ChIP assay with α-H3 acetylated in the presence and in the absence of AA treatment; n = 3 independent experiments, mean ± SD; unpaired Student’s t -test (two-tailed) ( t = 3.908, DF = 4) vs. Vehicle. Source data are provided as Source Data files.
Universal Methylated Human Dna Standard, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cpgenome human methylated & non-methylated dna standard sets s8001  (Merck KGaA)
90
Merck KGaA cpgenome human methylated & non-methylated dna standard sets s8001
CSB depletion by histone H3 hypoacetylation of its promoter. a Quantitative RT-qPCR of DNMT3A , DNMT3B , and DNMT1 . b <t>DNA</t> methylation (percent) of eight individual CpG sites in the CSB promoter at the indicated PN (positive control [CTL + ], a universal methylated (human) DNA standard). n = 3 independent experiments, mean ± SD; two-way ANOVA ( F = 2.983, DFn = 5, DFd = 96, p = 0.0151) with post-hoc Tukey’s test. c Immunoblots of H3 acetylated (H3Ac) and total histone H3 (reprobed after H3Ac stripping) at the indicated PN, with GAPDH as a loading control. d Quantitative RT-qPCR of HDAC1 and HDAC2 . e Quantitative PCR of DNA in a ChIP assay with either α-H3 acetylated or total α-H3 (material not available at PN35). Primers detect the occupancy of H3Ac/H3 at a specific region of the CSB promoter. Results are expressed as the percentage of input DNA normalized to H3 occupancy. f Scheme indicating the anacardic acid (AA) target. g Quantitative RT-qPCR of CSB upon treatment with increasing concentrations of AA or DMSO (Vehicle) for 24 h, and in untreated control (Untreated). h Immunoblot of H3 acetylated, total H3 histone, CSB, HTRA3, HTRA2, and p21 from whole-cell extracts with increasing concentrations of AA or DMSO (Vehicle) for 24 h, and 24 h after AA withdrawal and in the corresponding controls (Untreated). Samples on the same blot are framed; each frame displays the respective GAPDH used as a loading control (GAPDH (F-C staining in the middle blot)). Quantitative PCRs: n = 3 independent experiments; mean ± SD; one-way ANOVA ( a DNMT1 : F = 9.129, DFn = 5, DFd = 12, p = 0.0009; DNMT3A : F = 0.05094, Dfn = 5, DFd = 12, p = 0.9980; DNMT3B : F = 0.5325, DFn = 5, DFd = 12, p = 0.7481; d HDAC1 : F = 12.93, DFn = 5, DFd = 12, p = 0.0002; HDAC2 : F = 3.826, DFn = 5, DFd = 12, p = 0.0264; e H3: F = 2.298, DFn = 4, DFd = 10, p = 0.1304; H3Ac/H3: F = 5.064, DFn = 4, DFd = 10, p = 0.0171; g F = 9.516, DFn = 4, DFd = 10, p = 0.0019) with post-hoc Tukey’s test vs. PN16 (or Vehicle, g ) when not specifically indicated. i Quantitative PCR analysis of a DNA fragment in the CSB promoter from ChIP assay with α-H3 acetylated in the presence and in the absence of AA treatment; n = 3 independent experiments, mean ± SD; unpaired Student’s t -test (two-tailed) ( t = 3.908, DF = 4) vs. Vehicle. Source data are provided as Source Data files.
Cpgenome Human Methylated & Non Methylated Dna Standard Sets S8001, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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universal methylated human dna standard  (Zymo Research)
95
Zymo Research universal methylated human dna standard
CSB depletion by histone H3 hypoacetylation of its promoter. a Quantitative RT-qPCR of DNMT3A , DNMT3B , and DNMT1 . b <t>DNA</t> methylation (percent) of eight individual CpG sites in the CSB promoter at the indicated PN (positive control [CTL + ], a universal methylated (human) DNA standard). n = 3 independent experiments, mean ± SD; two-way ANOVA ( F = 2.983, DFn = 5, DFd = 96, p = 0.0151) with post-hoc Tukey’s test. c Immunoblots of H3 acetylated (H3Ac) and total histone H3 (reprobed after H3Ac stripping) at the indicated PN, with GAPDH as a loading control. d Quantitative RT-qPCR of HDAC1 and HDAC2 . e Quantitative PCR of DNA in a ChIP assay with either α-H3 acetylated or total α-H3 (material not available at PN35). Primers detect the occupancy of H3Ac/H3 at a specific region of the CSB promoter. Results are expressed as the percentage of input DNA normalized to H3 occupancy. f Scheme indicating the anacardic acid (AA) target. g Quantitative RT-qPCR of CSB upon treatment with increasing concentrations of AA or DMSO (Vehicle) for 24 h, and in untreated control (Untreated). h Immunoblot of H3 acetylated, total H3 histone, CSB, HTRA3, HTRA2, and p21 from whole-cell extracts with increasing concentrations of AA or DMSO (Vehicle) for 24 h, and 24 h after AA withdrawal and in the corresponding controls (Untreated). Samples on the same blot are framed; each frame displays the respective GAPDH used as a loading control (GAPDH (F-C staining in the middle blot)). Quantitative PCRs: n = 3 independent experiments; mean ± SD; one-way ANOVA ( a DNMT1 : F = 9.129, DFn = 5, DFd = 12, p = 0.0009; DNMT3A : F = 0.05094, Dfn = 5, DFd = 12, p = 0.9980; DNMT3B : F = 0.5325, DFn = 5, DFd = 12, p = 0.7481; d HDAC1 : F = 12.93, DFn = 5, DFd = 12, p = 0.0002; HDAC2 : F = 3.826, DFn = 5, DFd = 12, p = 0.0264; e H3: F = 2.298, DFn = 4, DFd = 10, p = 0.1304; H3Ac/H3: F = 5.064, DFn = 4, DFd = 10, p = 0.0171; g F = 9.516, DFn = 4, DFd = 10, p = 0.0019) with post-hoc Tukey’s test vs. PN16 (or Vehicle, g ) when not specifically indicated. i Quantitative PCR analysis of a DNA fragment in the CSB promoter from ChIP assay with α-H3 acetylated in the presence and in the absence of AA treatment; n = 3 independent experiments, mean ± SD; unpaired Student’s t -test (two-tailed) ( t = 3.908, DF = 4) vs. Vehicle. Source data are provided as Source Data files.
Universal Methylated Human Dna Standard, supplied by Zymo Research, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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universal methylated mouse dna standard  (Zymo Research)
95
Zymo Research universal methylated mouse dna standard
CSB depletion by histone H3 hypoacetylation of its promoter. a Quantitative RT-qPCR of DNMT3A , DNMT3B , and DNMT1 . b <t>DNA</t> methylation (percent) of eight individual CpG sites in the CSB promoter at the indicated PN (positive control [CTL + ], a universal methylated (human) DNA standard). n = 3 independent experiments, mean ± SD; two-way ANOVA ( F = 2.983, DFn = 5, DFd = 96, p = 0.0151) with post-hoc Tukey’s test. c Immunoblots of H3 acetylated (H3Ac) and total histone H3 (reprobed after H3Ac stripping) at the indicated PN, with GAPDH as a loading control. d Quantitative RT-qPCR of HDAC1 and HDAC2 . e Quantitative PCR of DNA in a ChIP assay with either α-H3 acetylated or total α-H3 (material not available at PN35). Primers detect the occupancy of H3Ac/H3 at a specific region of the CSB promoter. Results are expressed as the percentage of input DNA normalized to H3 occupancy. f Scheme indicating the anacardic acid (AA) target. g Quantitative RT-qPCR of CSB upon treatment with increasing concentrations of AA or DMSO (Vehicle) for 24 h, and in untreated control (Untreated). h Immunoblot of H3 acetylated, total H3 histone, CSB, HTRA3, HTRA2, and p21 from whole-cell extracts with increasing concentrations of AA or DMSO (Vehicle) for 24 h, and 24 h after AA withdrawal and in the corresponding controls (Untreated). Samples on the same blot are framed; each frame displays the respective GAPDH used as a loading control (GAPDH (F-C staining in the middle blot)). Quantitative PCRs: n = 3 independent experiments; mean ± SD; one-way ANOVA ( a DNMT1 : F = 9.129, DFn = 5, DFd = 12, p = 0.0009; DNMT3A : F = 0.05094, Dfn = 5, DFd = 12, p = 0.9980; DNMT3B : F = 0.5325, DFn = 5, DFd = 12, p = 0.7481; d HDAC1 : F = 12.93, DFn = 5, DFd = 12, p = 0.0002; HDAC2 : F = 3.826, DFn = 5, DFd = 12, p = 0.0264; e H3: F = 2.298, DFn = 4, DFd = 10, p = 0.1304; H3Ac/H3: F = 5.064, DFn = 4, DFd = 10, p = 0.0171; g F = 9.516, DFn = 4, DFd = 10, p = 0.0019) with post-hoc Tukey’s test vs. PN16 (or Vehicle, g ) when not specifically indicated. i Quantitative PCR analysis of a DNA fragment in the CSB promoter from ChIP assay with α-H3 acetylated in the presence and in the absence of AA treatment; n = 3 independent experiments, mean ± SD; unpaired Student’s t -test (two-tailed) ( t = 3.908, DF = 4) vs. Vehicle. Source data are provided as Source Data files.
Universal Methylated Mouse Dna Standard, supplied by Zymo Research, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bisulfite-converted universal methylated human dna standard  (Zymo Research)
93
Zymo Research bisulfite-converted universal methylated human dna standard
CSB depletion by histone H3 hypoacetylation of its promoter. a Quantitative RT-qPCR of DNMT3A , DNMT3B , and DNMT1 . b <t>DNA</t> methylation (percent) of eight individual CpG sites in the CSB promoter at the indicated PN (positive control [CTL + ], a universal methylated (human) DNA standard). n = 3 independent experiments, mean ± SD; two-way ANOVA ( F = 2.983, DFn = 5, DFd = 96, p = 0.0151) with post-hoc Tukey’s test. c Immunoblots of H3 acetylated (H3Ac) and total histone H3 (reprobed after H3Ac stripping) at the indicated PN, with GAPDH as a loading control. d Quantitative RT-qPCR of HDAC1 and HDAC2 . e Quantitative PCR of DNA in a ChIP assay with either α-H3 acetylated or total α-H3 (material not available at PN35). Primers detect the occupancy of H3Ac/H3 at a specific region of the CSB promoter. Results are expressed as the percentage of input DNA normalized to H3 occupancy. f Scheme indicating the anacardic acid (AA) target. g Quantitative RT-qPCR of CSB upon treatment with increasing concentrations of AA or DMSO (Vehicle) for 24 h, and in untreated control (Untreated). h Immunoblot of H3 acetylated, total H3 histone, CSB, HTRA3, HTRA2, and p21 from whole-cell extracts with increasing concentrations of AA or DMSO (Vehicle) for 24 h, and 24 h after AA withdrawal and in the corresponding controls (Untreated). Samples on the same blot are framed; each frame displays the respective GAPDH used as a loading control (GAPDH (F-C staining in the middle blot)). Quantitative PCRs: n = 3 independent experiments; mean ± SD; one-way ANOVA ( a DNMT1 : F = 9.129, DFn = 5, DFd = 12, p = 0.0009; DNMT3A : F = 0.05094, Dfn = 5, DFd = 12, p = 0.9980; DNMT3B : F = 0.5325, DFn = 5, DFd = 12, p = 0.7481; d HDAC1 : F = 12.93, DFn = 5, DFd = 12, p = 0.0002; HDAC2 : F = 3.826, DFn = 5, DFd = 12, p = 0.0264; e H3: F = 2.298, DFn = 4, DFd = 10, p = 0.1304; H3Ac/H3: F = 5.064, DFn = 4, DFd = 10, p = 0.0171; g F = 9.516, DFn = 4, DFd = 10, p = 0.0019) with post-hoc Tukey’s test vs. PN16 (or Vehicle, g ) when not specifically indicated. i Quantitative PCR analysis of a DNA fragment in the CSB promoter from ChIP assay with α-H3 acetylated in the presence and in the absence of AA treatment; n = 3 independent experiments, mean ± SD; unpaired Student’s t -test (two-tailed) ( t = 3.908, DF = 4) vs. Vehicle. Source data are provided as Source Data files.
Bisulfite Converted Universal Methylated Human Dna Standard, supplied by Zymo Research, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human methylated & non-methylated (wga) dna set (dna w/ primers)  (Zymo Research)
94
Zymo Research human methylated & non-methylated (wga) dna set (dna w/ primers)
CSB depletion by histone H3 hypoacetylation of its promoter. a Quantitative RT-qPCR of DNMT3A , DNMT3B , and DNMT1 . b <t>DNA</t> methylation (percent) of eight individual CpG sites in the CSB promoter at the indicated PN (positive control [CTL + ], a universal methylated (human) DNA standard). n = 3 independent experiments, mean ± SD; two-way ANOVA ( F = 2.983, DFn = 5, DFd = 96, p = 0.0151) with post-hoc Tukey’s test. c Immunoblots of H3 acetylated (H3Ac) and total histone H3 (reprobed after H3Ac stripping) at the indicated PN, with GAPDH as a loading control. d Quantitative RT-qPCR of HDAC1 and HDAC2 . e Quantitative PCR of DNA in a ChIP assay with either α-H3 acetylated or total α-H3 (material not available at PN35). Primers detect the occupancy of H3Ac/H3 at a specific region of the CSB promoter. Results are expressed as the percentage of input DNA normalized to H3 occupancy. f Scheme indicating the anacardic acid (AA) target. g Quantitative RT-qPCR of CSB upon treatment with increasing concentrations of AA or DMSO (Vehicle) for 24 h, and in untreated control (Untreated). h Immunoblot of H3 acetylated, total H3 histone, CSB, HTRA3, HTRA2, and p21 from whole-cell extracts with increasing concentrations of AA or DMSO (Vehicle) for 24 h, and 24 h after AA withdrawal and in the corresponding controls (Untreated). Samples on the same blot are framed; each frame displays the respective GAPDH used as a loading control (GAPDH (F-C staining in the middle blot)). Quantitative PCRs: n = 3 independent experiments; mean ± SD; one-way ANOVA ( a DNMT1 : F = 9.129, DFn = 5, DFd = 12, p = 0.0009; DNMT3A : F = 0.05094, Dfn = 5, DFd = 12, p = 0.9980; DNMT3B : F = 0.5325, DFn = 5, DFd = 12, p = 0.7481; d HDAC1 : F = 12.93, DFn = 5, DFd = 12, p = 0.0002; HDAC2 : F = 3.826, DFn = 5, DFd = 12, p = 0.0264; e H3: F = 2.298, DFn = 4, DFd = 10, p = 0.1304; H3Ac/H3: F = 5.064, DFn = 4, DFd = 10, p = 0.0171; g F = 9.516, DFn = 4, DFd = 10, p = 0.0019) with post-hoc Tukey’s test vs. PN16 (or Vehicle, g ) when not specifically indicated. i Quantitative PCR analysis of a DNA fragment in the CSB promoter from ChIP assay with α-H3 acetylated in the presence and in the absence of AA treatment; n = 3 independent experiments, mean ± SD; unpaired Student’s t -test (two-tailed) ( t = 3.908, DF = 4) vs. Vehicle. Source data are provided as Source Data files.
Human Methylated & Non Methylated (Wga) Dna Set (Dna W/ Primers), supplied by Zymo Research, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


CSB depletion by histone H3 hypoacetylation of its promoter. a Quantitative RT-qPCR of DNMT3A , DNMT3B , and DNMT1 . b DNA methylation (percent) of eight individual CpG sites in the CSB promoter at the indicated PN (positive control [CTL + ], a universal methylated (human) DNA standard). n = 3 independent experiments, mean ± SD; two-way ANOVA ( F = 2.983, DFn = 5, DFd = 96, p = 0.0151) with post-hoc Tukey’s test. c Immunoblots of H3 acetylated (H3Ac) and total histone H3 (reprobed after H3Ac stripping) at the indicated PN, with GAPDH as a loading control. d Quantitative RT-qPCR of HDAC1 and HDAC2 . e Quantitative PCR of DNA in a ChIP assay with either α-H3 acetylated or total α-H3 (material not available at PN35). Primers detect the occupancy of H3Ac/H3 at a specific region of the CSB promoter. Results are expressed as the percentage of input DNA normalized to H3 occupancy. f Scheme indicating the anacardic acid (AA) target. g Quantitative RT-qPCR of CSB upon treatment with increasing concentrations of AA or DMSO (Vehicle) for 24 h, and in untreated control (Untreated). h Immunoblot of H3 acetylated, total H3 histone, CSB, HTRA3, HTRA2, and p21 from whole-cell extracts with increasing concentrations of AA or DMSO (Vehicle) for 24 h, and 24 h after AA withdrawal and in the corresponding controls (Untreated). Samples on the same blot are framed; each frame displays the respective GAPDH used as a loading control (GAPDH (F-C staining in the middle blot)). Quantitative PCRs: n = 3 independent experiments; mean ± SD; one-way ANOVA ( a DNMT1 : F = 9.129, DFn = 5, DFd = 12, p = 0.0009; DNMT3A : F = 0.05094, Dfn = 5, DFd = 12, p = 0.9980; DNMT3B : F = 0.5325, DFn = 5, DFd = 12, p = 0.7481; d HDAC1 : F = 12.93, DFn = 5, DFd = 12, p = 0.0002; HDAC2 : F = 3.826, DFn = 5, DFd = 12, p = 0.0264; e H3: F = 2.298, DFn = 4, DFd = 10, p = 0.1304; H3Ac/H3: F = 5.064, DFn = 4, DFd = 10, p = 0.0171; g F = 9.516, DFn = 4, DFd = 10, p = 0.0019) with post-hoc Tukey’s test vs. PN16 (or Vehicle, g ) when not specifically indicated. i Quantitative PCR analysis of a DNA fragment in the CSB promoter from ChIP assay with α-H3 acetylated in the presence and in the absence of AA treatment; n = 3 independent experiments, mean ± SD; unpaired Student’s t -test (two-tailed) ( t = 3.908, DF = 4) vs. Vehicle. Source data are provided as Source Data files.

Journal: Nature Communications

Article Title: CSB promoter downregulation via histone H3 hypoacetylation is an early determinant of replicative senescence

doi: 10.1038/s41467-019-13314-y

Figure Lengend Snippet: CSB depletion by histone H3 hypoacetylation of its promoter. a Quantitative RT-qPCR of DNMT3A , DNMT3B , and DNMT1 . b DNA methylation (percent) of eight individual CpG sites in the CSB promoter at the indicated PN (positive control [CTL + ], a universal methylated (human) DNA standard). n = 3 independent experiments, mean ± SD; two-way ANOVA ( F = 2.983, DFn = 5, DFd = 96, p = 0.0151) with post-hoc Tukey’s test. c Immunoblots of H3 acetylated (H3Ac) and total histone H3 (reprobed after H3Ac stripping) at the indicated PN, with GAPDH as a loading control. d Quantitative RT-qPCR of HDAC1 and HDAC2 . e Quantitative PCR of DNA in a ChIP assay with either α-H3 acetylated or total α-H3 (material not available at PN35). Primers detect the occupancy of H3Ac/H3 at a specific region of the CSB promoter. Results are expressed as the percentage of input DNA normalized to H3 occupancy. f Scheme indicating the anacardic acid (AA) target. g Quantitative RT-qPCR of CSB upon treatment with increasing concentrations of AA or DMSO (Vehicle) for 24 h, and in untreated control (Untreated). h Immunoblot of H3 acetylated, total H3 histone, CSB, HTRA3, HTRA2, and p21 from whole-cell extracts with increasing concentrations of AA or DMSO (Vehicle) for 24 h, and 24 h after AA withdrawal and in the corresponding controls (Untreated). Samples on the same blot are framed; each frame displays the respective GAPDH used as a loading control (GAPDH (F-C staining in the middle blot)). Quantitative PCRs: n = 3 independent experiments; mean ± SD; one-way ANOVA ( a DNMT1 : F = 9.129, DFn = 5, DFd = 12, p = 0.0009; DNMT3A : F = 0.05094, Dfn = 5, DFd = 12, p = 0.9980; DNMT3B : F = 0.5325, DFn = 5, DFd = 12, p = 0.7481; d HDAC1 : F = 12.93, DFn = 5, DFd = 12, p = 0.0002; HDAC2 : F = 3.826, DFn = 5, DFd = 12, p = 0.0264; e H3: F = 2.298, DFn = 4, DFd = 10, p = 0.1304; H3Ac/H3: F = 5.064, DFn = 4, DFd = 10, p = 0.0171; g F = 9.516, DFn = 4, DFd = 10, p = 0.0019) with post-hoc Tukey’s test vs. PN16 (or Vehicle, g ) when not specifically indicated. i Quantitative PCR analysis of a DNA fragment in the CSB promoter from ChIP assay with α-H3 acetylated in the presence and in the absence of AA treatment; n = 3 independent experiments, mean ± SD; unpaired Student’s t -test (two-tailed) ( t = 3.908, DF = 4) vs. Vehicle. Source data are provided as Source Data files.

Article Snippet: Universal methylated human DNA standard (Enzo Life Sciences, ENZ-45005-0001) was use as a positive control.

Techniques: Quantitative RT-PCR, DNA Methylation Assay, Positive Control, Methylation, Western Blot, Stripping Membranes, Real-time Polymerase Chain Reaction, Staining, Two Tailed Test